In the 1980s, there were many domestic products of ELISA testers, which were mainly divided into two categories: one was continuous micro-sampling, produced by Shanghai No. 3 Analytical Instrument Factory, Jilin Siping Radio Factory, Zhejiang Wenzhou Mold Factory; The whole micro-reaction plate is put into the tester for measurement, and is produced by Sichuan Ninth Instrument Factory in Chongqing. Most domestic ELISA testers can meet the prescribed standards.
There is also a product that adds an MB-1 type enzyme-linked plug (492nm) to a type 72 spectrophotometer.
The ELISA tester is based on the color reaction between the enzyme and the related substrate to form an optical density change. It is designed and manufactured using the principle of photoelectric colorimetry. Some people in China call the ELISA tester "enzyme label colorimeter" or "enzyme label quantitative tester" "In fact, this is inaccurate, because academically" enzyme labeling "refers to the process by which enzymes are combined with antibody (or antigen) proteins in a certain way.
1. How to use the ELISA tester: The ELISA tester should be installed in a dry, clean, and quiet laboratory with relatively stable temperature and humidity. The power supply voltage can be used with alternating current. Use the time-division calibration instrument, test samples, and wash the cuvette. Link.
Calibrate the instrument: 1. Close the light door, insert the required filter, and turn the range selection switch to T "100%". 2. Turn on the power, the indicator light is visible. 3. Preheat for more than 15 minutes. 4. Adjust the instrument from the "0" switch to the meter pointer to 0.5, inject the control solution, open the light door, and adjust the dimming amount knob to make the meter pointer 100% full. 6. Turn the range to the desired A (light (Density) Range, use A knob to adjust to 0.
Determination of samples: 1. Install the pump button and remove the control solution. 2. Put down the button switch and inject the sample with a micro pipette (also called micro liquid sampler) to read the sample. The sample test procedure is from thin to concentrated .
Cleaning the cuvette: turn on the pump and clean the cuvette more than 3 times before testing samples with different components
Second, the auxiliary equipment related to the measurement of the ELISA tester: In the ELISA measurement, other related auxiliary use polystyrene vinyl plastic micro-reaction plates and micro-liquid samplers. In the 1980s, polystyrene plastic micro-reaction plates were produced by Shanghai Produced by the third plastics factory, the micro-liquid samplers of different volumes are produced by Shanghai Qiujing Glass Instrument Factory and other manufacturers. The quality of the polystyrene plastic micro-reaction plate is closely related to the ELISA test results, especially when using Sichuan Ninth Instrument Factory The ELISA tester produced is more prominent.
With the rapid development of science and technology in the 21st century, the performance and appearance of ELISA testers have undergone major changes.
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