Coating temperature and time After adding a certain amount of protein coating solution to the polyethylene carrier, most are placed in a 4.C refrigerator overnight to allow the solid surface to fully adsorb, or incubate with 37C for 2h to achieve the same adsorption effect, but 37C Coated with 2` ~ 3h and 1h, the difference between the last measured absorbance values ​​is not significant. The kinetic study of protein adsorption on polystyrene enzyme plate shows that: 1MIN after adding the coating solution, adsorption will occur on the solid phase, the amount of adsorption is related to protein concentration and coating time. When the protein concentration is 5ug / mL, the coating 60MIN has obvious adsorption. After 12h, the adsorption amount no longer increases with time. When the protein concentration is 10ug / mL and 20ug / mL, the adsorption of the two is similar, and the coating is 3min. There will be obvious adsorption, the adsorption is complete after 6h, the adsorption capacity in 5min is 30% of 6min
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