Sample dilution
Solid and semi-solid samples: Weigh 25 g of sample into a sterile homogenized cup containing 225 mL of phosphate buffer or saline, and homogenize for 1 min to 2 min at 8000 r/min to 10000 r/min. Or put into a sterile homogenized bag containing 225 mL of phosphate buffer or normal saline, and beat with a tapping homogenizer for 1 min to 2 min to make a 1:10 sample homogenate.
Liquid sample: Pipette 25 mL of the sample into a sterile conical flask containing 225 mL of phosphate buffer or saline (pre-positioned with appropriate amount of sterile glass beads) and mix thoroughly. 1:10 sample homogenization. Or put into a sterile homogenized bag containing 225 mL of phosphate buffer or normal saline, and tap for 1 min to 2 min with a tapping homogenizer to make a 1:10 sample homogenate.
The pH of the sample homogenate should be between 6.5 and 7.5, if necessary with 1 mol/L NaOH or 1 mol/L HCl.
Pipette 1 mL of 1:10 sample with 1 mL sterile pipette or micropipette, and slowly inject 9 mL of phosphate buffer or saline into the sterile tube along the tube wall (note that the tip of the pipette or tip does not touch Dilute the test tube, shake the tube or use a 1 mL sterile pipette repeatedly to mix and mix to make a 1:100 sample.
According to the estimation of the contamination state of the sample, the 10-fold incremental serial dilution sample homogenate was sequentially prepared according to the above operation. For each incremental dilution, replace with a 1 mL sterile pipette or tip. From the preparation of the sample homogenate to the completion of the sample inoculation, the entire process must not exceed 15 min.
Zhengzhou Weier Biotechnology Co., Ltd. is a comprehensive enterprise integrating scientific research, production and sales. The company's business covers biological agents, chemical reagents and chemical raw materials, pre-clinical testing and consumables, elisa kits, antibodies and standards. A variety of biological products such as products and culture media. Complete range, quality is trustworthy, welcome new and old customers to come to inquire!!!
Electric Hand Air Pump,Volleyball Air Pump,Portable Ball Air Pump,Ball Air Compressor
SHENZHEN SMARTNEWO TECHNOLOGY CO,. LTD , https://www.newopump.com