Gram-negative bacilli, aerobic or facultative anaerobic, optimal growth temperature of 35 ~ 37 ° C, the optimum pH value of 6.8 ~ 7.8. Does not break down lactose (except Arizona). Decomposes glucose to produce acid (the Salmonella typhimurium produces no acid). Most of them produce H2S, do not produce ruthenium matrix, do not liquefy gelatin, do not decompose urea, do not produce acetylmethylmethanol, most of them can use citrate, can reduce nitrate to nitrite, and do not grow on the medium.
Trisaccharide iron test
The test sample was inoculated with trisaccharide iron agar and cultured at 36±1° C. for 18 h to 24 h, and the results were observed.
The Salmonella reaction is as follows: the alfalfa produces alkali, the bottom produces acid, and produces H2S.
Reaction principle: Salmonella does not ferment lactose, so that the slope is red, fermenting glucose, and the bottom layer is yellow, and can decompose sulfur amino acids in the medium to form H2S. H2S forms black lead sulfide or iron sulfide precipitates by lead or iron ions. Things.
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